Friday, May 7, 2021
Mitochondrial Health

Axonal trafficking of mitochondria in primary neuron from a wild type mouse



The laboratory of Eugenia Trushina, Ph.D., is focused on mitochondrial dysfunction. For more information: http://www.mayo.edu/research/faculty/trushina-eugenia-ph-d/bio-00027310

Visualization of mitochondria in E17 hippocampal neuron was done using mitochondria-specific dye, TMRM. 600 frames were acquired by imaging the axon every second using LSM 510 confocal microscope. Imaging was done focusing on the axon with the cell body located at the top of the image. Resulting movie was analyzed using Analyze, a comprehensive multidimensional medical image processing, visualization and analysis software package developed by the Biomedical Imaging Resource of the Mayo Clinic. By treating the microscope image sequence as a spatial stack of cross-sectional images, the volume rendering algorithms in Analyze produce a 3D digital kymograph, allowing the motion of multiple organelles over a period of time to be visualized in a single static 2D image. Final kympgraph allows tracing each mitochondrion through all 600 frames to generate a final profile of movement. This application allowed demonstrating that inhibition of axonal trafficking precedes the development of multiple neurodegenerative diseases providing potential therapeutic targets.

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