A useful technique for studying cell structure and function
is cell fractionation which takes cells apart and separates major organelles and other subcellular structures
from one another. The piece of equipment that is used
for this task is the centrifuge, which spins test tubes holding
mixtures of disrupted cells at a series of increasing speeds.
At each speed, the resulting force causes a subset of the cell
components to settle to the bottom of the tube, forming a
pellet. At lower speeds, the pellet consists of larger components,
and higher speeds result in a pellet with smaller
Cell fractionation enables researchers to prepare specific
cell components in bulk and identify their functions, a task
not usually possible with intact cells. For example, on one
of the cell fractions, biochemical tests showed the presence
of enzymes involved in cellular respiration, while electron
microscopy revealed large numbers of the organelles called
mitochondria. Together, these data helped biologists determine
that mitochondria are the sites of cellular respiration.
Biochemistry and cytology thus complement each other in
correlating cell function with structure.
36 thoughts on “Cell fractionation | Basic technique of Cell biology | biology lecture”
Thank you Sir
Excellent teaching of topic…thankyou sir
Thank you sir 🙏
Sir ma na ap sa whole fsc karma ka erda karleya ma app ko ramzan ma aftare ka waqt special dua sa yad karu ga awr jab doctor ban jaya to pir ap muft elaj b karwoga awr sath agr paisau ke zarurt ha to bathna
Carry on with it bro…. btw who is in matric and decided to study fsc and now here??
Pls correct your information… It will be 3 hr in the last step. Neither 1hr nor 2hr.
Thanks Sir may you live long
Sir you had skip lecture on cell and cell theory and its discovery please make video on 11 class firsf chapter first topic. Mehrabane hoge sir
Sir so sweet lecture
Walekum Salam from India thank you
Sir what is ur good name ?
Hd dy pa eman.
apka name saad h kya
Thank you for your wonderful presentation sir
Thank u sir ur lacture is simple and very helpful 👍
Apki waja sey shayad mai acadmy join na karo thanks Alot of sir !!!!!
Thank you sir
I am your new subscriber thank u sir you helped me.
Amazing lecture sir
sir you so much resemble to phy with M arafat 😝
بہت اچهے ہوتے ہیں وہ لوگ جو بغیر کسی غرض کے کسی کو سپورٹ کرتے ہیں یا اللّٰہ تبارک وتعالی مجھے سپورٹ کرنے والوں کے والدین کو لمبی زندگی صحت کاملہ کیساتھ عطاء فرمائے آمین ثم آمین
you are such an amazing and hardworking man May Allah bless u Eveverywhere inn sha Allah 👌🤲❤
Awesome sir tysm 😍❤️🙏🏻🙏🏻🙏🏻🙏🏻
Very very nice lecture
Sir centripetal force Nahi hots centrifugal force hotha hai.or
tell me a rmp and g difference
Assalamualikum… jazakallahu khair … lecture is superb bro