Genevieve Van de Bittner, R&D Researcher at Agilent Research Laboratories, gave the January MIT.nano Seminar: “Measuring metabolic flux with cellular and molecular resolution: Combining Seahorse XF and LC/MS technologies for comprehensive analyses.”
Alterations in metabolic flux have been linked to a wide variety of cell processes, from cancer development to T cell activation during immunotherapy or infection. This talk covered two complementary technologies for measuring metabolic flux— Seahorse XF analyzers and liquid chromatography-mass spectrometry (LC/MS) qualitative flux analysis.
Both technologies provide important insights into the rates of metabolic reactions within cells, with Seahorse XF measurements providing cellular resolution and LC/MS qualitative flux measurements providing molecular resolution. Combined, these technologies offer an in-depth, multi-scale method for examining metabolic flux.
This presentation highlighted vignettes of combined Seahorse XF and LC/MS qualitative flux analyses that provide a comprehensive view of adipocyte metabolism during cool-temperature adaptation, metabolic alterations during infectious disease, mutation-specific metabolic flux in cancer cells, and the metabolic response of cancer cells to oxidative phosphorylation modulating compounds.