Thursday, December 8, 2022
Mitochondrial Health

जीनोम सीक्वेंसिंग क्या होता है ।। जीनोम सीक्वेंसिंग का पूरा प्रोसेस । #tricknowtech #Abdul

#Tricknowtech #Abdul
Whole genome sequencing (WGS), also known as full genome sequencing, complete genome sequencing, or entire genome sequencing, is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism’s genome at a single time. This entails sequencing all of an organism’s chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.

Electropherograms are commonly used to sequence portions of genomes.

An image of the 46 chromosomes, making up the diploid genome of human male. (The mitochondrial chromosome is not shown.)
Whole genome sequencing has largely been used as a research tool, but was being introduced to clinics in the future of personalized medicine, whole genome sequence data may be an important tool to guide therapeutic intervention.The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.

Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases.In addition, whole genome sequencing should not be confused with methods that sequence specific subsets of the genome – such methods include whole exome sequencing (1-2% of the genome) or SNP genotyping (0.1% of the genome). As of 2017 there were no complete genomes for any mammals, including humans. Between 4% to 9% of the human genome, mostly satellite DNA, had not been sequenced.

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