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High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells – a 2 minute Preview of the Experimental Protocol
Siamak Djafarzadeh, Stephan M. Jakob
University of Bern, Department of Intensive Care Medicine, Inselspital, Bern University Hospital;
High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.
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