๐๐จ๐ข๐ง ๐จ๐ฎ๐ซ “๐๐๐๐ ๐๐๐๐๐๐ ๐๐๐๐๐๐๐๐๐ ๐๐๐๐๐๐”
๐๐จ๐ซ
๐ ๐๐๐ฐ ๐๐๐ญ๐๐ก๐๐ฌ ๐๐จ๐ซ ๐๐๐๐ ๐๐๐ ๐๐ข๐๐ ๐๐๐ข๐๐ง๐๐๐ฌ
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๐๐ฅ๐๐๐ฌ๐ ๐ฏ๐ข๐ฌ๐ข๐ญ: ๐ฐ๐ฐ๐ฐ.๐ญ๐ซ๐ข๐ฒ๐๐ฆ๐๐๐ค.๐จ๐ซ๐
๐๐๐ฅ๐ฅ ๐๐ญ: +๐๐-๐๐๐๐๐๐๐๐๐๐, +๐๐-๐๐๐๐๐๐๐๐๐๐, +๐๐-๐๐๐๐๐๐๐๐๐๐
00:00 Splicing
5:25 P-TEFb activates elongation, splicing and polyadenylation and phosphorylates
Serine 2 CTD
TAT-SF1
SPT5
6:33 Ser2 phosphatase Fcp1 regenerates RNA Pol II pool
10:45 Splicing is an SN2 reaction that proceeds through a pentavalent phosphorous intermediate.
13:54 There are three type of Splicing Machinery
20:11 The group I and group II introns have the remarkable ability to excise themselves from an RNA. This is called auto-splicing, or self-splicing.
Group I introns are more common than group II introns.
23:53 Two transesterification reactions takes place for 2 exon -1 intron configuration. First transesterification is catalysed variably either by external G or branch point while second transesterification is always caused by exon 1 -3โOH.
29:40 There are three type of Splicing signals
Major U2-type or GU-AG introns
Minor U12-type or AU-AC introns
Minor U12-type with GU-AG introns (hybrid type)
37:22 Major U2-type or GU-AG introns
38:15 Splicing signals of minor introns
42:42 Minor U12-type with GU-AG introns (hybrid type)
42:44 Splice sites are directional in nature. 5โ splice site -the left, or donor, site and the 3โ splice site – right, or acceptor, site.
43:28 Splicing complex
51:15 Lariat formation is characterize by 2โ-5โ phosphodiester bond
53:21 Group II mitochondrial introns are excised by the same mechanism as nuclear pre-mRNAs via a lariat that is held together by a 2โโ5โ bond.
54:08 SR proteins are involved in exon definition in which they interact with U1 and U2AF forming commitment E complex.
57:32 U11 base pairs with the 5โ splice sites; U12 base pairs with the branch point sequence near the 3โ splice site; and U4atac and U6atac provide analogous functions during the spliceosome assembly and activation of the spliceosome.
U5 is same component in all splicing complexes
58:37 Each snRNP contains a single snRNA (100-300 nt) and several (20) proteins.
The U4 and U6 snRNPs are usually found together as a di-snRNP (U4/U6) particle.
1:00:01 U1 snRNA consist of four hairpins, one intron pairing strands and one sm binding domains.
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