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Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue – a 2 minute Preview of the Experimental Protocol
Chloé J Simard, Guillaume Pelletier, Luc H Boudreau, Etienne Hebert-Chatelain, Nicolas Pichaud
Université de Moncton, Département de chimie et biochimie; Université de Moncton, Département de biologie;
In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.
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